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. Author manuscript; available in PMC: 2020 Jan 5.
Published in final edited form as: Eur J Pharmacol. 2018 Oct 25;842:351–364. doi: 10.1016/j.ejphar.2018.10.028

Fig. 6A-G. Low-affinity/high-capacity transporter cell line comparison of [3H]MPP+ uptake inhibition by compounds 1–7 (A-G.).

Fig. 6A-G.

Competition of 10 nM [3H]MPP+ uptake inhibition in human PMAT- (⭕, solid line), OCT2- (■, solid line), and OCT3-HEK (□, dotted line) expressing cell lines. Concentrations of unlabeled competing D22 analogs ranged from 0 nM (control) up to 100 μM and are plotted on a log-scale. Uptake inhibition assays were performed at room temperature for 10 min. Data are fit to a non-linear regression analysis to determine IC50 estimations (see Table 4).