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. 2019 Mar 22;10:520. doi: 10.3389/fimmu.2019.00520

Figure 2.

Figure 2

DCs interact with T cells inside lymphatic capillaries and short interactions are I-A/I-E-independent in CHS-inflamed mouse ear skin. (A–F) Intravital microscopy was performed in CHS-inflamed ear skin of hCD2-DsRed×Prox1-GFP mice after adoptive transfer of DeepRed-labeled WT or I-A/I-E−/− BM-DCs. (A) Schematic diagram of the experimental setup. (B) Time-lapse images of a DeepRed+ WT DC (DC, cyan) contacting DsRed+ T cells (T1 and T2) inside a lymphatic capillary (scale bars: 30 μm). Times are shown in min. (C) Speed of WT and I-A/I-E−/− DCs within lymphatic capillaries. (D) Plots of contact between WT and I-A/I-E−/− DCs and T cells inside lymphatic capillaries. Each line is a DC indicating contact (green) and no contact (gray) with T cells. WT = 69 DCs, 174 contacts; I-A/I-E−/− = 77 DCs, 196 contacts. (E) Quantitative analysis of contact times from (C) are shown individually and after classification into three contact time groups. Median is shown as a red bar. (F) The occupancy of DCs by T cells from (C) are shown individually and after classification into three groups. Each dot in (C,E,F) represents a tracked cell. Medians are shown as red bars. Pooled data from 6 mice per group are shown.