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. 2019 Mar 1;23(2):106–111. doi: 10.1080/19768354.2019.1585948

Figure 2.

Figure 2.

Treatment with LRRK2 kinase inhibitor suppresses the TNFα release in Myc-G2019S LRRK2-expressed BV2 cells. (A) After 6 h transfection, Vector or myc-GS cells were incubated with or without GSK2578215A in the fresh growth medium for 36 h. To validate the LRRK2 transfection and inhibition by GSK2578215A, cell lysates were analyzed using anti-myc and pS1292 antibody. (B) The culture medium was collected and concentrated using a 3 K filtration tube, and the medium was subjected to mouse TNFα ELISA. Two-way analysis of variance (ANOVA) using Tukey’s multiple comparison test was used for the statistical analysis; ****p < 0.0001.