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. 2019 Mar;60(4):1275–1285. doi: 10.1167/iovs.18-24398

Figure 3.

Figure 3

Microglia-dependent Hcar2 expression. (A) Gene expression changes of Hcar2, Cd14, and Mrc1 from 1 to 24 hours after LIR. LIR upregulated Hcar2 and Cd14 from 1 to 24 hours after LIR. Mrc1 was decreased from 1 to 6 hours after LIR, but was increased from 12 to 24 hours after LIR. MMF treatment significantly suppressed the upregulations of Hcar2 and Cd14. Mean ± SE was reported at each time point, n = 3. (B) Whole mount retina staining with Iba1 showed that with 2 weeks of PLX5622, 77.6% of retinal microglia was depleted. (C) Fold regulation changes of Hcar2, Cd14, and Mrc1 due to microglia depletion. Depletion of microglia with PLX5622 reduced Hcar2 by 3.6-fold in the neural retina without LIR, and attenuated Hcar2 upregulation by LIR from 13.7- to 3.8-fold (12 hours after LIR). PLX5622 also reduced Cd14 (3.3- and 3.4-fold) and Mrc1 (8.1- and 24.3-fold) without LIR and with LIR, respectively. Mean ± SE was reported, n = 3.