Table 1.

qPCR Primer Sequences and Conditions

Gene	Primer (5′ to 3′)	Annealing Temp (°C)	Amplicon Size (bp)
Hcar2
Fwd	AGCATCATCTTCCTCACCGT	56	192
Rev	ACACAGATATGCCTCGCCAT
Cd14
Fwd	GAAGCAGATCTGGGGCAGTT	58	107
Rev	CGCAGGGCTCCGAATAGAAT
Mrc1
Fwd	GCAAACATTGGGCAGAAGGA	58	191
Rev	CGCCAGCTCTCCACCTATAG
Nfkb1
Fwd	GAGTCACGAAATCCAACGCA	58	217
Rev	AATCCCATGTCCTGCTCCTT
Nlrp3
Fwd	ATGCTGCTTCGACATCTCCT	58	196
Rev	AACCAATGCGAGATCCTGAC
Casp1
Fwd	CGTGGAGAGAAACAAGGAGTG	56	192
Rev	AATGAAAAGTGAGCCCCTGAC
Il-1β
Fwd	TGCCACCTTTTGACAGTGATG	54	136
Rev	ATGTGCTGCTGCGAGATTTG
Nrf2
Fwd	CTACTCCCAGGTTGCCCAC	56	241
Rev	CTGCCAAACTTGCTCCATGT
Hmox1
Fwd	ACAGAAGAGGCTAAGACCGC	60	238
Rev	ACAGGAAGCTGAGAGTGAGG
Gclc
Fwd	AAGCCTCCTCCTCCAAACTC	60	186
Rev	GGGCCACTTTCATGTTCTCG
Nqo1
Fwd	ATGCTGCCATGTACGACAAC	60	168
Rev	AGACCTGGAAGCCACAGAAA
Eef2
Fwd	TGTCAGTCATCGCCCATGTG	60	123
Rev	CATCCTTGCGAGTGTCAGTGA
Tbp
Fwd	TGACCCCTATCACTCCTGCC	60	250
Rev	TGTTCTTCACTCTTGGCTCCTG