Table 6.
Non-viral vectors used in gene therapy for genetic hearing loss studies
| Non-viral vector | Example | Load | Animal | Route of administration | Reference |
|---|---|---|---|---|---|
| Cationic liposomes | Liposomes | β-gal plasmid | Guinea pig | RWM after cochleostomy | [287] |
| Liposomes | eGFP plasmid | Mouse | Gelfoam on RWM | [288–290] | |
| Lipofectamine 2000 | Math1 | Rat | OC-derived cell line | [291] | |
|
| |||||
| Cationic non-liposomal polymers | Polybrene | Integrin antisense oligonucleotide | Rats | OC-derived cell line | [292] |
| Dendritic polymers (HPNP) | eGFP plasmid | Rat | Sponge on RWM/cochlear explants | [293] | |
| Polyethylenimine (PEI) | eGFP plasmid | Guinea pig | Scala tympani injection | [294] | |
| PLGA nanoparticles | Fluorescent dye (Rhodamine) | Guinea pig | Gelfoam on RWM | [295] | |
|
| |||||
| Biolistic | Gold particles using Gene gun | MyoXVa | Mouse | OC explants | [296, 297] |
|
| |||||
| Electroporation | Electroporation | Math1 | Rat | OC explants | [298, 299] |
| Electroporation | Math1 | Mouse | In utero | [300, 301] | |
RWM, round window membrane; eGFP, enhanced green fluorescent protein; OC, organ of Corti; PLGA, poly(lactic-co-glycolic acid).