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. Author manuscript; available in PMC: 2019 Mar 29.
Published in final edited form as: Methods Mol Biol. 2015;1329:203–213. doi: 10.1007/978-1-4939-2871-2_16

Fig. 1.

Fig. 1

Analysis of peak fractions eluted from Superdex 200 gel filtration column by SDS-PAGE. Aliquots (10 μL) of peak fractions and side fractions were subjected to SDS-PAGE on an 8–16 % Tris–glycine minigel. The peak fractions (lanes 1–5) were highly enriched in the BAM holocomplex. These fractions were pooled and used to generate proteoliposomes. As illustrated here, the side fractions (lanes 6–8) often appear to contain incomplete BAM complexes