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. 2018 Oct 18;4(12):1742–1748. doi: 10.1001/jamaoncol.2018.4292

Figure 2. Quantification of Phosphorylated Histone (γ-H2AX) Staining of Skin From Unirradiated Mice (Controls) Compared With Skin From Mice Irradiated With 15 Gy ×1 in the Presence or Absence of Skin Ointment of Varying Thickness.

Figure 2.

Quantification of γ-H2AX staining in frozen sections of skin from unirradiated mice (control) compared with skin sections from mice irradiated with 15 Gy ×1 in the presence or absence of petroleum-based ointment (petrolatum, 41% [Aquaphor; Beiersdorf AG]) of varying thickness, including application of 50 mg of ointment (moderate application) and application of 200 mg of ointment (thick application). Quantification of γ-H2AX–positive cells per 10× field at 8 hours (A) and 24 hours (B) after 15 Gy of RT in immunofluorescent images is presented. Data are expressed as mean; whiskers indicate SEM (n = 3). RT indicates radiotherapy. To convert gray to rad, multiply by 100.