Figure 3. Quantification of Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling (TUNEL) Staining in Frozen Sections of Skin From Control Mice Compared With Skin Sections From Mice Irradiated to 15 Gy ×1 in the Presence or Absence of Skin Ointment of Varying Thickness.

Quantification of positively stained TUNEL apoptotic cells in frozen sections of skin from unirradiated mice (control) compared with skin sections from mice irradiated to 15 Gy ×1 in the presence or absence of petroleum-based ointment (petrolatum, 41% [Aquaphor; Beiersdorf AG]) of varying thickness at 8 hours (A) and 24 hours (B) after 15 Gy ×1, including application of 50 mg of ointment (moderate application) and application of 200 mg of ointment (thick application). Data are expressed as mean; whiskers indicate SEM (n = 3). RT indicates radiotherapy. To convert gray to rad, multiply by 100.