Table 3.
Summary of symbiotic performance of Venezuelan isolates of soybean-nodulating bacteria
| Strain Code | Site (Ecosystem)a | Soil type | MLST analysis | nod genesb | nifH | Root nodule numbersc | ARAd |
|---|---|---|---|---|---|---|---|
| VA11 | Aragua (V-WF) | Inceptisol | R. pusense | B. japonicum | R. pusense | 21±3.0 | 64.1±1.5 |
| VA35 | R. tropici | B. diazoefficiens | R. pusense | 48±3.1 | 68.4±2.0 | ||
| VA1011-2A | Bradyrhizobium sp. | Bradyrhizobium sp. | B. japonicum | 35±1.6 | 25.8±4.3 | ||
| VA107A | R. pusense | B. japonicum | B. japonicum | 24±6.0 | 44.2±5.0 | ||
| VA107B | R. pusense | B. japonicum | B. japonicum | 20±1.5 | 44±4.4 | ||
| VA109-3B | Bradyrhizobium sp. | Bradyrhizobium sp. | B. japonicum | 41±4.4 | 40.2±4.0 | ||
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| VA312 | P. phytofirmans | P. fungorum | B. elkanii | 24.7±4.0 | 51.3±3.0 | ||
| VA109-3 | *B. zhejiangensis | *Burkholderia sp. | *Burkholderia sp. | 41±8.6 | 40.2±5.1 | ||
| VA109-3A | P. fungorum | P. fungorum. | *Burkholderia sp. | 42±7.0 | 23.5±3.0 | ||
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| VAF110B | Aragua (V-F) | Inceptisol | R. alamii | Bradyrhizobium sp. | Bradyrhizobium sp. | 22±2.8 | 24±1.0 |
| VAF123A | Bradyrhizobium sp. | Bradyrhizobium sp. | B. japonicum | 17.6±3.0 | 25.3±1.3 | ||
| VAF123B | Bradyrhizobium sp. | Bradyrhizobium sp. | B. japonicum | 19±4.0 | 26±1.0 | ||
| VAF124 | R. pusense | B. diazoefficiens | R. pusense | 38±1.7 | 52.1±4.0 | ||
| VAF1243 | R. pusense | B. diazoefficiens | R. pusense | 34±3.7 | 31.4±4.2 | ||
| VAF125 | R. pusense | B. diazoefficiens | R. pusense | 18±0.5 | 66.4±2.9 | ||
| VAF1269 | Bradyrhizobium sp. | Bradyrhizobium sp. | B. japonicum | 22±1.5 | 49.4±1.8 | ||
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| VAF32B | *Burkholderia sp. | *Burkholderia sp. | B. elkanii | 42.7±5.5 | 55.8±0.5 | ||
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| VAm17A | Amazonas (Am-r) | Oxisol | R. tropici | Bradyrhizobium sp. | R. pusense | 50±1.5 | 78.5±1.2 |
| VAm13A | Bradyrhizobium sp. | Bradyrhizobium sp. | R. pusense | 40±1.0 | 57.2±0.24 | ||
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| VAm18B | *B. zhejiangensis | *Burkholderia sp. | Rhizobium sp. | 45±4.5 | 77.6±1.2 | ||
| VAm22 | *Burkholderia sp. | B. elkanii | B. elkanii | 30±2.9 | 42.9±0.8 | ||
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| VAn112 | Anzoategui (S-NC) | Ultisol | B. lupini | B. diazoefficiens | B. diazoefficiens | 23.3±1.5 | 50.5±0.9 |
| VAn113 | B. diazoefficiens | B. diazoefficiens | B. diazoefficiens | 22±1.8 | 43.2±3.9 | ||
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| VAp110 | Apure (F) | Inceptisol | P. phytofirmans | P. fungorum | B. elkanii | 35±1.5 | 58.3±4.0 |
| VAp116 | P. phytofirmans | B. elkanii | B. elkanii | 58±3.1 | 69.9±2.0 | ||
| VAp15 | P. phytofirmans | B. elkanii | B. elkanii | 70±5.9 | 74.7±0.7 | ||
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| VDC12 | DC (N.P-C) | Alfisol | *B. zhejiangensis | *Burkholderia sp. | *Burkholderia sp. | 17±1.5 | 26±0.3 |
| VDC15 | *Burkholderia sp. | B. elkanii | *Burkholderia sp. | 25±3.2 | 32±1.9 | ||
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| VF23 | Falcon (A-D) | Aridisol | *Burkholderia sp. | *Burkholderia sp. | B. elkanii | 47.8±4.3 | 53.3±1.4 |
| VF24 | *Burkholderia sp. | *Burkholderia sp. | *Burkholderia sp. | 46.4±2.1 | 45±1.0 | ||
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| VG10B | Guarico (S) | Vertisol | *Burkholderia sp. | *Burkholderia sp. | *Burkholderia sp. | 38±2.5 | 57.1±2.0 |
| VG7 | *Burkholderia sp. | *Burkholderia sp. | *Burkholderia sp. | 25.3±2.5 | 32±1.1 | ||
| VG9 | P. phytofirmans | B. elkanii | B. elkanii | 35±3.3 | 44.4±0.9 | ||
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| VLa18 | Lara (X-NS) | Vertisol | R. pusense | Bradyrhizobium sp. | B. elkanii | 28.7±1.4 | 53.3±2.1 |
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| VLa19 | *Burkholderia sp. | B. elkanii | B. elkanii | 21±1.5 | 64±1.9 | ||
| VLa21 | P. phytofirmans | *Burkholderia sp. | B. elkanii | 26±1.9 | 34±3.0 | ||
| VLa31B | P. phytofirmans | *Burkholderia sp. | B. elkanii | 33±5.0 | 51±1.4 | ||
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| VMi21 | Miranda (M) | Alfisol | *Burkholderia sp. | *Burkholderia sp. | Rhizobium sp. | 29±2.2 | 40±2.1 |
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| VTr19-2B | Trujillo (A) | Ultisol | R. alamii | Bradyrhizobium sp. | B. japonicum | 35±1.5 | 83.6±1.0 |
| VTr19A | R. alamii | Bradyrhizobium sp. | R. alamii | 30±2.5 | 51.19±1.9 | ||
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| VTr19B | P. phytofirmans | *Burkholderia sp. | Rhizobium sp. | 55.3±4.9 | 43.8±2.7 | ||
| VTr28 | P. phytofirmans | B. elkanii | B. elkanii | 25±2.0 | 34.5±3.0 | ||
| VTr32 | P. phytofirmans | B. elkanii | B. elkanii | 58±5.0 | 62.9±4.1 | ||
| VTr35 | P. phytofirmans | B. elkanii | B. elkanii | 68±6.8 | 86.6±2.5 | ||
Sequences were compared with Blast in GenBank. MLST: multilocus sequence typing analysis (See the description of primers). B.: Bradyrhizobium.
B.: Burkholderia
V-F: valley with fertilizer; S-NC: Xerophilic ecosystem near the coast; X-NS: xerophilic near the savanna; V-WF: valley without fertilizer; Am-r: Amazon-rainforest; A-D: arid-like desert; A: Andes. N.P-C: National Park inside the city.
Results are based on sequences for Alphaproteobacteria with the nodD gene and intergenic spacer nodDB on Betaproteobacteria (See the description of primers for nodulation)
Results are the numbers of nodules per plant 4 weeks after the inoculation (mean standard deviations; n=3). Control plants (non-inoculated) had no nodules. The plant test was performed with G. max cultivar INIA
Acetylene reduction assay (ARA). Values represent activity expressed as nmol C2H4−1 h−1 g−1 dry weight of nodules.