Table 2.
Oxidation of various epoxyalkanes by recombinant EaCoMT in PE-TB08W
| Substrates | Relative residual substrates (%) | |
|---|---|---|
|
| ||
| Control | Recombinant EaCoMT | |
| Epoxypropane | 89.4±1.11 | 62.6±1.39 |
| (R)-(+)-epoxypropane | 92.8±0.12 | 28.0±0.73 |
| (S)-(+)-epoxypropane | 90.2±0.54 | 55.1±0.41 |
| Epoxyethane | 96.2±0.22 | 15.2±0.21 |
| 1,2-epoxybutane | 100.4±0.20 | 99.6±0.20 |
| cis-2,3-epoxybutane | 91.9±0.25 | 78.7±0.10 |
| trans-2,3-epoxybutane | 105.9±0.07 | 96.9±0.24 |
Epoxypropane contains (R)-(+)-epoxypropane and (S)-(−)-epoxypropane. Since epoxyethane is a liquid, 1.2 mL (5 μmol) of epoxyethane was injected into a vial. One-hundred-milliliter samples were injected into a gas chromatographic system. Data are mean values in duplicate represented as means±standard deviations.