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. 2019 Mar 26;8:e43531. doi: 10.7554/eLife.43531

Figure 3. The premature differentiation of the external gill-associated cranial muscle complex in the Senegal bichir larva.

Figure 3.

(A–C) Dorsal view on bichir embryos, developing skeletal muscles are revealed by 12/101 antibody (red). The red signal in A (st. 27) refers to the post-otic somites. The first cranial muscle is associated with the external gills (B, stage 29). (C) Superimposed fluorescent and SEM image showing the context of the external gill muscles. (D) Transversal section through the external gills at the level indicated in B. DAPI (blue) stains cell nuclei. (E, F) Stage 30 bichir embryo, lateral view with (E) cranial nerves fibres labeled with anti-acetylated tubulin, and with (F) cranial muscles stained with 12/101 antibody (red). (G) Stage 33 bichir embryo, lateral view, with developed cranial muscles stained with 12/101 antibody (red). Asterisk, cement gland; am, adductor mandibulae; ah/ao, complex of adductor hyomandibulae and adductor operculi; b, brain; ba, branchial arches; bm, branchiomandibularis; cd,constrictor dorsalis; cement gland; e, eye primordium; lb/db, complex of levator branchiarum and depressor branchiarum; hh, hyohyoideus; ih, interhyoideus; im, intermandibularis; ot, otic vesicle; pf, pectoral fin; y, yolk; V., nervus trigeminus; VII., nervus facialis.