Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | anti-BrdU; BrdU antibody (Rat monoclonal) |
Abcam | Cat#ab6326 | (1:200) |
| Antibody | anti-CD31; CD31 antibody (Rat monoclonal) |
Dianova | Cat#DIA-310 | (1:100) |
| Antibody | anti-GFP; GFP antibody (Chicken monoclonal) |
Aves Labs | Cat#GFP-1020 | (1:500) |
| Antibody | anti-4-Hydroxynonenal; 4-HNE antibodyl (Rabbit polyclonal) |
Abcam | Cat#ab46545 | (1:75) |
| Antibody | anti-NRF2; NRF2 antibody (Rabbit polyclonal) |
Abcam | Cat#ab137550 | (1:600) |
| Antibody | anti-Glutathione Peroxidase 1; GPX1 antibody (Rabbit polyclonal) |
Abcam | Cat#ab22604 | (1:200) |
| Antibody | Goat polyclonal anti-chicken |
Thermo Fisher | Cat#A-11039 | (1:1000) |
| Antibody | Goat polyclonal anti-rat |
Thermo Fisher | Cat#A-11006 | (1:1000) |
| Antibody | Goat polyclonal anti-mouse | Abcam | Cat#ab150117 | (1:1000) |
| Biological sample (Human) |
Human breast cancer lung metastases tissue microarray (TMA) |
This paper (Section of lung tissue containing cancer cells was surgically removed from breast cancer patients, preserved in paraflim and a small portion of the preserved tumor was used to make the TMA) |
N/A | Tissue microarray Available from Edi Brogi |
| Chemical compound, drug |
Doxycycline | Sigmal-Aldrich | Cat#D9891 | |
| Chemical compound, drug |
5-Fluorocytosine; 5-FC | Sigma-Aldrich | Cat#F7129 | |
| Chemical compound, drug |
5-Fluorouracil; 5-FU | Sigma-Aldrich | Cat#F6627 | |
| Chemical compound, drug |
SB-505124 | Sigma-Aldrich | Cat#S4696 | |
| Chemical compound, drug |
Thymine | Sigma-Aldrich | Cat#T0376 | |
| Chemical compound, drug |
4-Thiouracil; TU | Sigma-Aldrich | Cat#440736 | |
| Other | Oligo (dT)25 magnetic beads |
New England Biolabs | Cat#S1419S | |
| Other | Protein G Dynabeads | Thermo Fisher Scientific |
Cat#10004D | |
| Commercial assay or kit |
Tissue digestion C-tube |
Miltenyi | Cat#130-096-334 | |
| Commercial assay or kit |
Mouse Tumor Dissociation Kit |
Miltenyi | Cat#130-096-730 | |
| Commercial assay or kit |
TruSeq RNA Library Prep Kit v2 |
Illumina | RS-122–2001 | |
| Commercial assay or kit |
SMARTer PCR cDNA synthesis kit |
Clontech | Cat#634926 | |
| Commercial assay or kit |
Nextera XT DNA library Preparation Kit |
Illumina | FC-131–1024 | |
| Commercial assay or kit |
RNeasy MinElute Cleanup kit |
Qiagen | Cat#74204 | |
| Commercial assay or kit |
cDNA kit-First Strand Transcriptor |
Roche | Cat#043790–12001 | |
| Cell line (Human) | MDA231 | Laboratory of Joan Massague |
PMID: 19421193 | Expresses TGL |
| Cell line (Human) | MDA231-CD | This paper (MDA231 cells were transduced with rtTA3 and TRE-CD-IRES-RFP) |
N/A | Available from Massague lab |
| Cell line (Human) | MDA231-CD/UPRT | This paper (MDA231 cells were transduced with rtTA3 and TRE-UPRT-T2A-RFP-IRES-CD) |
N/A | Available from Massague lab |
| Cell line (Human) | 293T | Laboratory of Joan Massague |
N/A | |
| Strain, strain background (Mus musculus) |
Hsd:Athymic Nude- Foxn1nu |
Envigo | Cat#069 | |
| Sequence-based reagents |
Oligonucleotides | This paper | N/A | Oligonucleotide sequences are provided in Supplementary file 6 |
| Recombinant DNA reagent |
CMV Tight RFP-IRES-CD | This paper (RFP-IRES-CD was subcloned into CMV Tight EGFP Puro (Addgene: Plasmid #26431) vector by removing EGFP). |
N/A | Available from Massague lab |
| Recombinant DNA reagent |
CMV Tight UPRT- T2A-RFP-IRES-CD |
This paper (UPRT-T2A-RFP-IRES-CD was subcloned into CMV Tight EGFP Puro (Addgene: Plasmid #26431) vector by removing EGFP). |
N/A | Available from Massague lab |
| Recombinant DNA reagent |
rtTA3 | Addgene | Plasmid #26730 | |
| Software and Algorithms |
STAR2.5.2b | PMID: 23104886 | https://github.com/alexdobin/STAR | |
| Software and Algorithms |
HTSeq v0.6.1p1 | PMID: 20979621 | https://htseq.readthedocs.io/en/release_0.10.0/ | |
| Software and Algorithms |
DESeq2 v3.4 | PMID: 25516281 | https://bioconductor.org/packages/release/bioc/html/DESeq2.html | |
| Software and Algorithms |
GSVA v3.4 | PMID: 23323831 | https://bioconductor.org/packages/release/bioc/html/GSVA.html |