TABLE 1.
Detection of fungi using f-ITS2-PCR-CEa
| Materialb | No. (%) of samples |
||||
|---|---|---|---|---|---|
| Total analyzed | Negative | Positive | Monofungalc | Polyfungalc | |
| C | 127 | 97 (76.4) | 30 (23.6) | 26 (86.7) | 4 (13.3) |
| U-C | 101 | 60 (59.4) | 41 (40.6) | 30 (73.2) | 11 (26.8) |
| Sd | 127 | 111 (87.4) | 16 (12.6) | 16 (100) | 0 (0) |
| U-S | 64 | 45 (70.3) | 19 (29.7) | 17 (89.5) | 2 (10.5) |
| Total | 419 | 313 (74.8) | 106 (25.2) | 89 (84) | 17 (16) |
The table shows an overview of molecular detection in clinical samples. C. robusta detected in sonication fluids (material C and S) was evaluated as a contaminant and was excluded from the results. These results also include three non-Candida fungal species, identified by sequencing as Geotrichum candidum, Rhodotorula rubra, and Cryptococcus neoformans, in 2 U-C, 1 U-C, and 1 polyfungal U-C sample, respectively. Candida was detected in 8 samples (3 C. parapsilosis, 3 C. albicans, 1 C. tropicalis, and 1 C. famata isolate) out of 29 and 2 (C. albicans) out of 3 catheter and stent samples without a corresponding urine sample, respectively. In 3 U-C cases without a corresponding catheter sample, no Candida was detected.
C, urinary catheter; U-C, urine from patients with catheter; S, ureteral stent; U-S, urine from patients with stent.
Percentage of positive samples.
Data for the stent category are based on the results of 127 unique samples. In the disjunction of results of proximal and distal tip samples, there were 14 (20.9%) Candida sp. positive stents; no stent was polyfungal.