TABLE 4.
Comparison of culture and f-ITS2-PCR-CE results by using a higher inoculation volume for culture (100 μl)
| Result type | Culture method result |
f-ITS2-PCR-CE result |
No. (%) of positive samplesb
|
|||||
|---|---|---|---|---|---|---|---|---|
| Identificationa | No. (%) of samples tested | Identificationa | No. (%) of samples tested | C | U-C | S | U-S | |
| Concordant | Negative | 43 | Negative | 43 | 23 | 11 | 7 | 2 |
| C. tropicalis | 5 | C. tropicalis | 5 | 3 | 2 | |||
| C. albicans | 6 | C. albicans | 6 | 2 | 1 | 2 | 1 | |
| C. parapsilosis | 2 | C. parapsilosis | 2 | 1 | 1 | |||
| Partially concordant | C. albicans > Candida sp. | 2 | C. albicans | 2 | 1 | 1 | ||
| C. albicans > Candida sp. | 1 | C. albicans > C. parapsilosis | 1 | 1 | ||||
| Candida sp. | 2 | C. parapsilosis | 2 | 1 | 1 | |||
| Candida sp. | 1 | C. parapsilosis > C. albicans | 1 | 1 | ||||
| C. tropicalis | 2 | C. tropicalis > C. glabrata | 2 | 1 | 1 | |||
| C. albicans > C. glabrata | 1 | C. parapsilosis > C. albicans | 1 | 1 | ||||
| Rhodotorula rubra | 1 | Non-Candida sp.c | 1 | 1 | ||||
| Discrepant | Negative | 16 | C. albicans | 3 | 2 | 1 | ||
| C. famata | 2 | 1 | 1 | |||||
| C. lusitaniae | 1 | 1 | ||||||
| C. parapsilosis | 5 | 1 | 4 | |||||
| C. robusta | 1 | 1 | ||||||
| C. famata > C. lusitaniae | 1 | 1 | ||||||
| C. parapsilosis > C. tropicalis | 1 | 1 | ||||||
| C. robusta > C. albicans | 1 | 1 | ||||||
| C. robusta > C. famata | 1 | 1 | ||||||
| Total | 82 | 82 | 38 | 31 | 9 | 4 | ||
| Negative | 59 (72) | 43 (52) | 23 | 11 | 7 | 2 | ||
| Positive | 23 (28.0) | 39 (47.6) | 15 (39.5) | 20 (64.5) | 2 (22.3) | 2 (50) | ||
| Polyfungald | 4 (17) | 9 (23) | ||||||
a
Species in the same line are in order of decreasing signal.
b
C, urinary catheter; U-C, urine from patient with catheter; S, stent; U-S, urine from patient with stent.
c
Identified as Rhodotorula rubra by sequencing.
d
Number (percentage) of polyfungal samples out of positive samples.