TABLE 1.

Compiled PCR results of the shared panel of S. flexneri strains^a

Expected serotype	No. of strains tested	No. of strains positive for gene with multiplex PCR									No. of strains positive for wzx6 with Sf6 PCR	Serotype result (n)	Differences between serology and PCR
		wzx1-5	gtrI	gtrII	oac	gtrIV	gtrV	gtrIC	gtrX	opt
1a	5	5	5	0	0	0	0	0	0	0	0	1a (5)	No difference
1b	4	4	4	0	4	0	0	0	0	0	0	1b (4)	No difference
1d	4	4	4	0	0	0	0	0	4	0	0	1d (4)	No difference
2a	5	5	0	5	0	0	0	0	0	0	0	2a (5)	No difference
2b	4	4	0	4	0	0	0	0	4	0	0	2b (4)	No difference
3a	3	3	0	0	3	0	0	0	3	0	0	3a (3)	No difference
3b	4	4	1(1)	0	4	0	0	0	0	0	0	3b (3), 1b (1)(1), (2)	(1) 1 strain type I (−) but gtrI (+) in 6 lab
													(2) 1 strain group 7,8 (+) but gtrX (−) in 1 lab
4a	3	3	0	0	0	3	0	0	0	0	0	4a (3)	No difference
4b	2	2	0	0	2	2	0	0	0	0	0	4b (1)	No difference
4av	2	2	0	0	0	2(3)	0	0	0	2	0	4av (2)	(3) 1 strain IV-2 (−) but gtrIV (+) in 1 lab
5a	8(4)	8	0	0	7(5)	0	8	0	2(6)	0	0	5a (1), 5a oac (+)(7)(5), 5b (2)(6)	(4) 2 strains 7,8 (+) but gtrX (−) in 1 lab
													(5) 7 strains FG6 (−) but oac (+) in all lab
													(6) 2 strains 7,8 (+) and gtrX (+) in 1 lab
5b	7	7	0	0	7(7)	0	7	0	7	0	0	5b oac (+) (7)(7)	(7) 7 strains FG6 (−) but oac (+)
6	4	0	0	0	0	0	0	0	0	0	4	6 (4)	No difference
7a (1c)	1	1	1	0	0	0	0	1	0	0	0	7a (1)	No difference
X	5	5	0	0	0	0	0	0	5	0	0	X (5)	No difference
Xv	3	3	0	0	0	0	0	0	3	3	0	Xv (3)	No difference
Y	5	5	0	0	0	0	0	0	1(8)	0	0	Y (4), X (1)	(8) 1 strain FG7,8 (−) but gtrX (+) in all lab
Yv	2	2	0	0	0	0	0	0	0	2	0	Yv (2)	No difference

^a(+), positive result; (−), negative result; lab, laboratory/laboratories. The superscript numbers in parentheses are defined in the far right column.