TABLE 3.

Assessment of assay performance in unequal triple mixtures: E. anophelis target lepA^a

Target sample (EkA) concn	Cq
	Target	EkA, 1.5 × 103; EkM, 1.5 × 101; RiA, 1.5 × 102	EkA, 1.5 × 103; EkM, 1.5 × 102; RiA, 1.5 × 101	EkA, 1.5 × 102; EkM, 1.5 × 103; RiA, 1.5 × 101	EkA, 1.5 × 102; EkM, 1.5 × 101; RiA, 1.5 × 103	EkA, 1.5 × 101; EkM, 1.5 × 103; RiA, 1.5 × 102	EkA, 1.5 × 101; EkM, 1.5 × 102; RiA, 1.5 × 103	SD
1.5 × 103	28.14	28.29	28.20					0.07
1.5 × 102	31.67			31.49	32.01			0.26
1.5 × 101	34.72					34.53	34.84	0.16

^aDNA extracts of E. anophelis (EkA), E. meningoseptica (EkM), and the most closely related species from the nearest genus within the family Flavobacteriaceae, Riemerella anatipestifer (RiA), in unequal mixtures of 1.5 × 10¹, 1.5 × 10², and 1.5 × 10³ GE/µl.