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. Author manuscript; available in PMC: 2020 Apr 15.
Published in final edited form as: Biol Psychiatry. 2018 Oct 25;85(8):667–678. doi: 10.1016/j.biopsych.2018.10.009

Figure 6: Microglial Hyperactivity to ex vivo LPS Stimulation in Stress-Sensitized Mice was Attenuated by Microglial Elimination and Repopulation.

Figure 6:

A) Male C57BL/6 mice were provided diets formulated with vehicle or CSF1R antagonist (PLX5622) for 14 days. Next, mice were stress-sensitized (SS) by RSD or left undisturbed as controls (Naϊve). After RSD-sensitization, all mice were provided vehicle diets for an additional 24 days to allow for repopulation of microglia. After 24 days of repopulation, microglia were collected by Percoll-enrichment and were cultured ex vivo with saline or LPS (100 ng/ml). mRNA levels of B) TLR4, CD14, D) IL-6, and E) IL-1β were determined in ex vivo microglia 4 h after LPS stimulation. Means with (*) are significantly different from Control-Naïve (p<0.05) and means with (#) are significantly different from SS-Naïve (p<0.05).