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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Breast Cancer Res Treat. 2019 Jan 3;174(3):605–613. doi: 10.1007/s10549-018-05079-7

Figure 3. Chk1 suppresses the induction of DSB in TNBC patient tumor biopsies.

Figure 3.

A, Representative images of ex vivo γ-H2AX foci in breast cancer patient tumor biopsies. Tumor biopsies were incubated under standard cell culture conditions and exposed to LY2603618 (100 nM) or mock treatment for 24h. Specimens were snap frozen 30 minutes after 1 Gy irradiation or mock treatment and analyzed by immunofluorescence microscopy and H&E staining.

B, Percentage of cells with LY2603618-induced γ-H2AX foci at 30 minutes post-irradiation in breast cancer explants. Bars represent mean ± standard error based on 8–10 random images and 200–400 nuclei per data point.