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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: J Thorac Oncol. 2018 Dec 19;14(4):656–671. doi: 10.1016/j.jtho.2018.12.004

Figure 5. GGA2 stabilizes EGFR levels after ligand activation.

Figure 5.

(A) NIH-3T3 cells expressing EGFRWT were infected with lentiviral constructs to stably overexpress GGA2 (GGA2 OE) or GFP leading to GGA2 overexpression in the former as indicated by Western blot. (B) GGA2 OE and GFP control cells were seeded in serum starved media before being subjected to 20uM cycloheximide for 30 minutes. Lysates were harvested for time point 0. Remaining cells were treated with 30ng/mL EGF and harvested at 1, 3, 6, 9, 12, 24 and 48 hours (h). Lysates were subjected to immunoblot analyses demonstrating that EGFR and pEGFR levels were more persistent in cells overexpressing GGA2. (C) The same experiment as described in (B), with 3ng/mL EGF also indicating increased EGFR stability and activity in the setting of GGA2 overexpresion.