Fig. 2.

Characterization and classification of DLBCLs from IPB mice. a Immunohistochemical characterization and sub-classification of mouse DLBCLs (n = 25) from ITP2-M;Rosa26^PB/+;Blm^m3/m3 (IPB) mice. Microscopic images of two representative DLBCL cases, one classified as germinal center B-cell like (GCB) DLBCL (left panel) and the other as non-GCB DLBCL (right panel). Tumors consist of large-sized neoplastic cells, show strong expression of B220/CD45R (B-cell marker), and are negative for CD138 (plasma cell marker). While the GCB DLBCL sample exhibits high expression of Bcl6 and is negative for Mum1/Irf4, the non-GCB DLBCL case shows the opposite expression pattern. Both tumors show high proliferation rates as indicated by Ki-67 immunohistochemistry (IHC). Scale bar, 50 µm. b RNA sequencing-based sub-classification of DLBCLs (n = 25) from IPB mice. For clustering of the expression data, mouse orthologues to human DLBCL classifier genes⁴⁰ were used. The heatmap shows z-transformed expression values. Two main clusters (A and B) were identified. IHC-based subtyping (GCB/non-GCB) of the corresponding tumors is indicated at the top of the heatmap. GEP gene expression profiling, ABC activated B-cell like