Fig. 6.

Loss of heterozygosity analysis in tumors from IPB mice. a Loss of heterozygosity (LOH) analysis workflow. Fragment of Pten containing the single nucleotide polymorphism (SNP) rs30424206 was amplified and sequenced in tumors and tails from ITP2-M;Rosa26^PB/+;Blm^m3/m3 (IPB) mice (n = 10). b Variant frequencies determined by Pten SNP sequencing of tail and tumor tissue samples from six IPB mice. All tumors harbored high-coverage Pten insertions. c Pten IHC  of tumors from IPB mice (n = 10), as well as from DLBCLs generated in a Blm-proficient screen using ATP2-S1;Rosa26^PB/+ mice (n = 7). Pten expression was scored semi-quantitatively. Representative microscopic images are shown. Pie charts display proportions of tumor samples with different Pten expression scores. Scale bar, 50 µm; Insets, ×200 magnification