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. 2019 Mar 29;10:1415. doi: 10.1038/s41467-019-09180-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A CRISPR/Cas9-based in vivo platform for BCL functional genomics. a Outline of the functional genomic approach for in vivo gene validation. b Kaplan–Meier plot showing survival of mice transplanted with hematopoietic stem/progenitor cells (HSPC) transduced with Trp53 sgRNA, Trp53 shRNA, and non-targeting (NT) sgRNA. p < 0.0001 for both Trp53-sgRNA vs. NT-sgRNA and Trp53-shRNA vs. NT-sgRNA. p < 0.03 for Trp53-sgRNA vs. Trp53-shRNA. p-values (log-rank test) were corrected for multiple testing using the Bonferroni method. c The transposon insertion pattern in Rfx7 predicts that gene-disruption is the cancer-causing mechanism. Each arrow represents an individual insertion. Insertions from all DLBCLs in the cohort are shown. ITP2 transposons can trap genes in either orientation. Insertions are distributed over the whole length of the gene (predicting a tumor suppressor). There is no bias for hot-spot areas of insertions as typically observed for unidirectional "activating" insertions in oncogenes. Consensus coding sequence (Rfx7-201) is displayed. d Kaplan–Meier plot for mice transplanted with HSPCs transduced with Rfx7 sgRNA and NT sgRNA. p < 0.0001, log-rank test. e Transposon insertion pattern in Phip, predicting tumor suppressive function of the gene. Consensus coding sequence (Phip-201) is shown. Each arrow represents an individual insertion. Insertions from all DLBCLs in the cohort are shown. f Heatmap displaying copy number variations on human chromosome 6 in samples from the TCGA-DLBC (n = 48) dataset (TCGA Research Network: http://cancergenome.nih.gov). The position of PHIP is indicated. g Function of Phip as a B-cell lymphoma tumor suppressor was validated using the CRISPR/Cas9-based in vivo functional genomic approach. Kaplan–Meier plot for mice transplanted with HSPCs transduced with Phip sgRNA and non-targeting (NT) sgRNA transplants. p < 0.0001, log-rank test. sgRNA single guide RNA, EFS elongation factor 1-alpha core promoter, eGFP enhanced green fluorescent protein, Mb megabase