Fig. 3.

Impaired spine maturation in hippocampal spines in Gprasp2 KO mice. a Representative images from secondary dendrites of CA1 hippocampal neurons from Gprasp2^−/y mice and WT controls. Scale bar 1 µm. b Reduced density of mature spines in secondary apical branches of hippocampal CA1 neurons from Gprasp2^−/y mice; WT n = 24/3 branches/mice, KO n = 24/3 branches/mice; two-tailed t-test. c Representative electron micrographs from hippocampal PSDs from Gprasp2^−/y and WT mice. Scale bar, 100 nm. d, e Gprasp2^−/y mice present thinner PSDs (d) but no significant alterations in PSD length (e) when compared with WT animals; WT n = 337/3 PSDs/mice; KO n = 362/3 PSDs/mice, two-tailed Mann–Whitney test. Data in (d) and (e) (main graph) are presented as a cumulative frequency plot, all other data are presented as means ± s.e.m. Statistical significance: *p < 0.05, **p < 0.01