Fig. 4.

Cell-autonomous reduction in dendritic complexity and spine density in acute GPRASP2 knockdown. a Cultured hippocampal neurons were transfected with scramble shRNA (Scramble), shRNA against Gprasp2 (shRNA) or GFP-GPRASP2 + shRNA (rescue) at DIV10 and analysed at DIV15. b Sholl analysis reveals reduced dendritic complexity in shRNA transfected neurons when compared with Scramble (effect of treatment **p < 0.01) and rescue (effect of treatment *p < 0.01); scramble n = 38, shRNA n = 30, rescue n = 37, from three independent preparations; two-way repeated measures ANOVA with Bonferroni post hoc test. c GPRASP2 knockdown decreases total dendritic length when compared with scramble and rescue transfected neurons; scramble n = 38, shRNA n = 30, rescue n = 37, from three independent preparations; one-way ANOVA test followed by Tukey multiple comparisons test. d–g GPRASP2 knockdown leads to decreased spine density (e), but no change in spine length (f) or head diameter (g) when comparing shRNA to scramble condition; scramble n = 24, shRNA n = 29, rescue n = 24, from three independent preparations; one-way ANOVA test followed by Tukey multiple comparisons test. h When divided into spine types, dendritic spine density was decreased across most spine types following GPRASP2 overexpression, but no changes are observed when normalized to fractional counts; scramble n = 24, shRNA n = 29, rescue n = 24, from three independent preparations; one-way ANOVA test followed by Tukey multiple comparisons test. Data are presented as means ± s.e.m. Statistical significance: *p < 0.05, **p < 0.01 and ***p < 0.001