Fig. 5.

GPRASP2 overexpression induces cell autonomous increased in spine density and spine maturation. a Cultured hippocampal neurons were transfected with GFP alone or GFP-GPRASP2 at DIV11 and analysed at DIV17. b Sholl analysis reveals increased dendritic complexity in GPRASP2-overexpressing neurons compared with GFP controls; GFP n = 31, GPRASP2 n = 31, from three independent preparations; two-way repeated measures ANOVA. c GPRASP2 overexpression increases total dendritic length; GFP n = 31, GPRASP2 n = 31, from three independent preparations; two-tailed t-test. d–g GPRASP2 overexpression leads to increased spine density (e), increased spine length diameter (f) and increased spine head diameter (f); GFP n = 31, GPRASP-GFP n = 28 from three independent preparations; two-tailed t-test. Scale bars 2 µm. h Dendritic spine density was increased following GPRASP2 overexpression, with the exception of filopodia-like spines, which showed a significant decrease; GFP n = 31, GPRASP-GFP n = 28 from three independent preparations; one-way ANOVA test followed by Tukey multiple comparisons test. Data are presented as means ± s.e.m. Statistical significance: *p < 0.05, **p < 0.01 and ***p < 0.001