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. 2019 Mar 29;9:5372. doi: 10.1038/s41598-019-41870-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ctgf response dynamics can be either transient or sustained. (A) Single cell ctgf traces grouped in transient or sustained response to 5 nM TGF-β stimulation with manual inspection. (B) Absolute and normalized single-cell traces from TGF-β (5 nM) stimulation experiments categorized into two classes using k-means clustering (n = 301 cells). (C) Silhouette plot of cells sorted according to ctgf expression dynamics. (D) Population-average of cells responding in a transient (blue) or sustained (red) manner. Solid lines: mean; shaded areas: SD.