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. 2019 Mar 29;9:5388. doi: 10.1038/s41598-019-41959-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Development of fluorescence in Brachycephalus ephippium. Three live individuals of B. ephippium share the same skin colour (a: natural lighting) but present different levels of fluorescence (b; UV illumination using two SANKYO lamps λ_excitation = 315–400 nm, no filter), corresponding to the extent of dermal ossification. The smallest individual (juvenile) does not present any fluorescent pattern; the larger juvenile presents several fluorescent points on its back and head; the adult presents the complete pattern, with more obvious fluorescence of the head and back. Photographs taken by S.G. and C.J.