Fig. 3.

Specific subsets of PDA_TME dendritic cell (DC) exhibit distinct phenotypic properties. a The gating strategy for subset analysis of PDA_TME and spleen DC based on expression of CD11b, CD103, and CD8α is depicted. b Frequencies of PDA_TME and spleen DC subsets based on expression of CD11b, CD103, and CD8α were analyzed. Representative contour plots and quantitative results are shown. Experiments were repeated >5 times. c Histogram plots comparing cytokine expression in distinct DC subsets are shown. Numbers adjacent to histograms represent MFI ÷ 10. Quantitative data based on MFI are shown. Dotted-line separates PDA_TME DC from splenic DC controls. Experiments were repeated three times. d The frequency of IL-6 and TGF-β1 co-expression in PDA_TME DC subsets was tested. Representative contour plots and quantitative data are shown. This experiment was repeated three times. e Enzymatic activity of ALDH in PDA_TME DC subsets was determined by the ALDEFLUOR assay. Gates were determined by respective DEAB controls (dotted histogram) for each individual sample. This experiment was repeated three times (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, t-test), SEM