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. 2019 Mar 29;9:5335. doi: 10.1038/s41598-019-41800-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Size distribution and Polydispersity index (PDI) analysis of using dynamic light scattering. To determine the size distribution, freshly isolated exosomes were subjected to dynamic light scattering measurements using DelsaMax Pro (Backman Coulter Inc.). Exosomes isolated using Ultracentrifugation, 101Bio (PureExo), MagCapture and iZON gel-filtration, were under the size range (<150 nm), while the exosomes isolated from Invitrogen precipitation method showed broader size distribution with a shift towards the bigger size (182 ± 13.93). Data presented as mean ± s.d.; n = 3.