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. 2019 Mar 29;9:5335. doi: 10.1038/s41598-019-41800-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Biological effect of isolated exosomes on cells. To assess the quality of exosomal preps for functional analyses, we used isolated exosomes (10 µg/ml) for the treatment of MiaPaCa cells (5 × 10³) grown in 96-well plate. After 72 h, the cell proliferation assay was performed using WST-1 reagent along with control without exosome treatment. As expected we observed some growth promoting effect on MiaPaCa cells of all exosomal preps except the one isolated using Invitrogen method, which exhibited some toxicity. Data presented as mean ± s.d.; n = 3, p-value *≤0.0117, and **≤0.0065.