Table 2.

Summery of different exosomes isolation methods.

Methods	Classical (Ultracentrifugation)	Invitrogen (Precipitation)	101 Bio (PureExo)	MagCapture (Affinity Based)	iZON (Size exclusion)
Principle	Based on density and size under centrifugal force	Differential solubility based precipitation	By precipitation and targeted filtration for removal of protein contamination	Affinity method using magnetic beads and phosphatidylserine (PS)-binding protein TIM4	Based on size exclusion chromatography
Pros	Good for exosomes treatment-based study, mass spectrometry analysis, RNA-seq study	Quick, easy and needs small sample size, high yield, good for small volume, RNA-seq study	Quick, easy, yield pure exosomes good for mass spectroscopy RNA-seq study	Single step, easy, no hoarse chemical, good for mass spectroscopy RNA-seq study	Fast, easy, single kit can be used for all type of sample, little volume is required
Cons	Time consuming, aggregated proteins and nucleic acid may be pelleted, not good for small volumes	Precipitates non- EV material, not good for mass spectrometry	Good for small volume only	Low yield,  good for small volume only	Low concentrated prep, additional method for enrichment  required
Time Required	3–4 h	12–16 h	1.5–2.0 h	4–5 h	1.5–2.0 h