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. 2019 Mar 29;9:5314. doi: 10.1038/s41598-019-41761-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Model system for studying Su(Hw)-dependent repression. (a) Genome browser view of insulator protein binding. The regions used in qPCR are indicated by vertical black arrows and numbered according to their chromosome position (FlyBase, 2006). The names of identified genomic regions are given next to the schemes of model genes. (b) ChIP-qPCR data on the binding of insulator proteins to the promoters in the ovaries and heads of females. ChIP was performed with antibodies against Su(Hw), Mod(mdg4)-67.2 (Mod-67.2, the C-terminal region corresponding to the specific isoform), CP190, Mod-com (the region common to all Mod(mdg4) isoforms), and nonspecific immunoglobulins (IgG, control). The ras64B coding region (ras) was used as a control devoid of Su(Hw) binding sites. The percent recovery of immunoprecipitated DNA (Y axis) was calculated relative to the amount of input DNA. Error bars indicate standard deviation of three independent biological replicates. Statistical analysis (Student’s t-test) was performed relative to protein binding to the ras64B coding region. Asterisks indicate significance levels of *p < 0.05, **p < 0.01, or ***p < 0.001 (here and in Figs 2–5).