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. 2019 Mar 29;9:5378. doi: 10.1038/s41598-019-41801-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mt/gDNA ratio in embryo culture medium corresponding to different time of blastulation. At D3, the mt/gDNA ratio corresponding to different time of blastulation in the embryo culture medium (a). Mt/gDNA ratio corresponding to different time of blastulation in D5 embryo culture medium (b). According to the time when embryos grow to the blastocyst stage, samples were divided into two groups, D5 and D6, such as the abscissa. The ordinate represents the ratio of mt/gDNA in the culture broth. One black dot or black square represents one sample. Data are means ± SEM.