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. 2019 Mar 29;9:5378. doi: 10.1038/s41598-019-41801-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mt/gDNA ratio in embryo culture medium corresponding to different age of woman (regardless of euploidy). At D3, the mt/gDNA ratio in the embryo culture medium corresponding to different age of woman (a). Mt/gDNA ratio corresponding to different time of blastulation in D5 embryo culture medium (b). According to the patient’s age, they were divided into three groups, which are less than or equal to 30 years old, greater than 31 years old and less than 34 years old and greater than or equal to 35 years old (<=30, 31–34 and >=35), such as the abscissa. The ordinate represents the ratio of mt/gDNA in the culture medium. Data are means ± SEM.