Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 29;10:1444. doi: 10.1038/s41467-019-09431-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Loss of Shp-2 leads to increased mature NK cell numbers. a, b Representative flow cytometric image, frequency, and number of splenic NK cells (gated as NK1.1⁺CD3/CD19⁻) of Ncr1^Ki Ptpn11^fl/fl (dark gray) and control mice (white) are depicted (a) or of splenic NK cells (gated as NK1.1⁺CD3⁻YFP⁺NKp46⁺) of Ncr1^Tg Ptpn11^fl/fl (light gray) or Ncr1^Tg Ptpn11^wt/fl (white) mice (b) are depicted. c, d Analysis of NK cells from Ncr1^Ki Ptpn11^fl/fl and control mice. c Representative cytometric plot of BM NK cells (gated as CD122⁺NK1.1⁺NKp46⁺CD3/CD19⁻) stained with CD27 and CD11b; percentage and number of CD27 single-positive (CD27 SP; CD27⁺CD11b⁻), double-positive (DP; CD27⁺CD11b⁺), and CD11b single-positive (CD11b SP; CD27⁻CD11b⁺) populations are shown. d Representative cytometric plot of splenic NK cells (gated as NK1.1⁺ NKp46⁺CD3/CD19⁻) stained with CD27 and CD11b; percentage and number of the afore-mentioned subsets are shown. e As in (d), but for NK cells gated as NK1.1⁺CD3⁻YFP⁺NKp46⁺ from Ncr1^Tg Ptpn11^fl/fl or Ncr1^Tg Ptpn11^wt/fl mice. f Frequency and number of CD27 SP, DP, and CD11b SP conventional NK cells (gated as CD45⁺NK1.1⁺CD3⁻YFP⁺NKp46⁺DX5⁺CD49a⁻) from Ncr1^Tg Ptpn11^fl/fl and control mice in the liver and a representative staining of CD27 and CD11b expression. a, b Results represent the mean ± SEM of n = 8 (Ncr1^Ki Ptpn11^fl/fl) and n = 13 (Ncr1^Ki Ptpn11^wt/wt) (a), n = 29 (Ncr1^Tg Ptpn11^wt/fl) and n = 35 (Ncr1^Tg Ptpn11^fl/fl) (b) mice per genotype, are a pool of three (a) and nine (b) experiments, which are representative of at least five (a) and eleven (b) independent experiments. c–f Results represent mean ± SEM of n = 4 (Ncr1^Ki Ptpn11^fl/fl) and n = 6 (Ncr1^Ki Ptpn11^wt/wt) (c), n = 7 (Ncr1^Ki Ptpn11^fl/fl) and n = 10 (Ncr1^Ki Ptpn11^wt/wt) (d), n = 29 (Ncr1^Tg Ptpn11^wt/fl) and n = 35 (Ncr1^Tg Ptpn11^fl/fl) (e), and n = 16 (Ncr1^Tg Ptpn11^wt/fl) and n = 20 (Ncr1^Tg Ptpn11^fl/fl) (f) mice per genotype, are a pool of two (d), nine (e, f) experiments and representative of at least three (c, d), and eleven (e, f) independent experiments. Statistical comparisons are shown; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, NS, non-significant; Student’s t-test. Source data are provided as a Source Data file