Fig. 5.

Ptpn11-deficient NK cells show a defective energetic response to IL-15. a, b Extracellular acidification rate (ECAR, a) and oxygen consumption rates (OCR, b) prior and after addition of the indicated mitochondrial inhibitors (oligomycin, trifluoromethoxy carbonylcyanide phenylhydrazone (FCCP), antimycin A, rotenone, and 2-deoxy-D-glucose (2DG) were measured for NK cells from Ncr1^Ki Ptpn11^wt/wt (blue) and Ncr1^Ki Ptpn11^fl/fl (red) mice cultured overnight in the presence of IL-15 (50 ng/ml). SRC (spare respiratory capacity). Results represent the mean ± SD of n = 6 (Ncr1^Ki Ptpn11^wt/wt) and n = 9 (Ncr1^Ki Ptpn11^fl/fl) replicates per genotype and are representative of at least three independent experiments. Statistical comparisons are shown on bar graphs; ****p ≤ 0.0001; Student’s t-test. Source data are provided as a Source Data file