Fig. 8.

Shp-2 mediates NK cell activation in response to IL-15 in vivo. a, b CPD-labeled splenic YFP⁺ NK cells from Ncr1^Tg Ptpn11^fl/fl (CD45.2⁺; light gray line and bars) mice were sorted and injected at a 1:1 ratio with sorted splenic NK cells from B6.SJL (CD45.1⁺; black line and white bars) congenic mice into recipient Rag2^−/−Il2Rg^−/− mice. Percentage of cells in divisions 1–3 or 4–7 (a) and relative proportion of total NK cells were determined at day 4 post-transfer (b). c, d Splenic YFP⁺ NK cells from Ncr1^Tg Ptpn11^fl/flROSA^EYFP (CD45.2⁺) mice were sorted and injected at a 1:1 ratio with sorted splenic NK cells from B6.SJL (CD45.1⁺) congenic mice into MCMV infected NK cell-deficient mice (Ncr1cre^KiROSA-DTA). Frequencies of total NK cells (c), and of Ly49H⁺ and Ly49H⁻ NK cells (d) were determined at day 7 post-infection. Results represent mean ± SEM of n = 3 mice (a, b) or of n = 13 mice (c, d), and are representative of three independent experiments (a, b) or a pool of three independent experiments (c, d). Statistical comparisons are shown; ***p ≤ 0.001, ****p ≤ 0.0001, NS, non-significant; Student’s t-test. Source data are provided as a Source Data file