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. 2019 Mar 29;9:5361. doi: 10.1038/s41598-019-41779-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Association of nerve profiles to the vasculature. (a–h) Images showing double immunostaining of CD34+ vasculature in combination with PGP9.5+ or TH+ nerve profiles (red arrows) in the bone marrow. Large arteries with PGP9.5+ (a) or TH+ (e) nerve profiles, small arteries with PGP9.5+ (b) or TH+ (f) nerve profiles, capillaries with PGP9.5+ (c) or TH+ (g) nerve profiles, and sinusoids with PGP9.5+ (d) or TH+ (h) nerve profiles. (i) The percentage distribution of PGP9.5+ and TH+ nerve profiles associated with the respective vascular structures in the bone marrow, and (j) the number of nerve profiles/cluster profile for the respective vascular structures. TH: tyrosine hydroxylase, PGP9.5: protein gene product 9.5; lAr: large arteriole; sAr: small arteriole; Ca: capillary; Nv: Nerve profiles with no association to the vasculature. n = 11.