Fig. 5.

PGK1 interacts directly with HSP90 and modulates the ATPase activity of HSP90. a. Western blot was performed to assess the effects of PGK1 knockdown on expression levels of HSP90 in Ishikawa cancer cell line. β-actin was used as a loading control. b. Semi-quantitative analysis of protein level in A. c. Western blot was performed to assess the effects of PGK1 knockdown on expression levels of HSP90 in HEC1A cancer cell line. β-actin was used as a loading control. d. Semi-quantitative analysis of protein level in C. e. Effect of PGK1 overexpression and HSP90 inhibitor 17-AAG on cellular ATP level. Vector, empty vector; PGK1, vector for PGK1 overexpression; PGK1 + 17-AAG, vector for PGK1 overexpression supplemented with 17-AAG. f. Co-immunoprecipitation was used to detect binding between PGK1 and HSP90. Hemagglutinin (HA)-tagged PGK1 (PGK1-HA) was overexpressed in Ishikawa cells. Data were shown as mean ± SD based on three independent experiments. *, P < 0.05, **, P < 0.01 compared with shNC, empty vector or PGK1 overexpressed (PGK1) group respectively