Table 2.

Variant Filtration Steps of sequencing and results of gene-based analysis

Filtration Steps	Number of variants
1.SNVsa located in exonic/ splicing region	188
2. Nonsynonymous SNVs/ frameshift deletion/ stopgain	125
3. Variants judged as SIFT prediction = Damaging / unknown and PolyPhen-2 prediction = Possibly damaging/ Probably damaging/ Unknown	71
4. MAF < 0.05 in 1KGP(Chinese Han population) and GeneSky in-house Database	42
5.Variants with sequenced base depth > 10x	18
Result of RNF213 gene-based analysis
MAF	PWSS	PSKAT	PSKAT-O
< 0.05	4.88 × 10−10	9.68 × 10−6	3.42 × 10−9
< 0.01	4.56 × 10−12	2.80 × 10−6	3.20 × 10−11

^aSNVs Single Nucleotide Variants