Fig. 5.

MiR-188-3p suppresses migration, invasion and proliferation in BCa cells. a miR-188-3p was shown to suppress the migration in MCF-7 and MDA-MB-231 cells, as evaluated by Transwell assay. **p < 0.01 compared with control sing independent sample T-test; scale bar, 200 μm. b Similarly, miR-188-3p was presented to inhibit the invasion of MCF-7 and MDA-MB-231 cells, as assayed by Transwell, too. **p < 0.01 compared with control using independent sample T-test. Scale bar, 200 μm. c miR-188-3p was displayed to significantly slow down the proliferation in MCF-7 and MDA-MB-231 cells using Clonogenic assay. ***p < 0.001 compared with control using independent sample T-test. Shown was representative figure picked out of three times of independent repeats; d miR-188-3p can pronouncedly induce the apoptosis in MCF-7 and MDA-MB-231 cells, as analyzed by Flow Cytometry. ***p < 0.001 compared with control group using independent sample T-test. e Xenograft nude mice model demonstrated that miR-188-3p can significantly inhibit the growth of MCF-7 cells in vivo. f Kinetic growth curve of tumor lesion were plotted. W week; g qRT-PCR detection of miR-188-3p expression level in tumor lesions dissected from xenograft nude mice, *p < 0.001 compared with control; h western-blot detection of variation of TMED3 in tumor lesions dissected. Shown was representative figure picked out of three times of independent repeats