Table 2.
Details of primers of Rubus ellipticus used for the present study
| Sr. no. | Primer name | Name of the gene | Primer sequence | Tm* (°C) | GC %* | Length in bp |
|---|---|---|---|---|---|---|
| 1. | R1 | AK317453.1(Actin gene) |
F: CTACGAGGGTTTCTCTCTTCCT R: CTGCTCGTAGTCAACAGCAAC |
54.8 54.4 |
50% 52% |
22 21 |
| 2. | R2 | Ethylene-responsive transcription factor 4 |
F: AACTTTACTTAGGTGCTCTCAACG R: TCATCAGCAGTACCATCAACAA |
59.3 56.5 |
41.7% 40.9% |
24 22 |
| 3. | R3 | MYB-related 306-like |
F: CTAGAACTCGAACCATTGTGGA R: GGGTTTTCAGGTAATTCACAGC |
58.4 58.4 |
45.5% 45.5% |
22 22 |
| 4. | R4 | WRKY transcription factor 35 |
F: TTGGATCAAGAAGGGACAAAAG R: CACATCCCCTGTGTTACTCTCA |
56.5 60.3 |
40.9% 50% |
22 22 |
| 5. | R5 | WRKY transcription factor 31 |
F: CCAAAACCCTGATTGACACTTT R: GCCATCTTCATTTGCTGATACA |
56.5 56.5 |
40.9% 40.9% |
22 22 |