Figure 1.

Propofol had no effect on CD86 mRNA and cell surface expression in M1 macrophages. M0 macrophages were polarized to M1 macrophages in the presence of 0.05% DMSO (solvent control) or propofol (1–5 μM). (a) qRT-PCR assays of CD86 mRNA levels. Data were normalized relative to β-actin mRNA (internal control) and presented as mean ± SD (n = 3 per group). (b) Flow cytometric analysis of CD86 surface expression on M1 macrophages treated with propofol (thick line) or DMSO (thin line) and on M1 macrophages incubated with isotype-matched control IgG and propofol (gray-filled line) or DMSO (dashed line).