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. 2019 Mar 17;2019:1919538. doi: 10.1155/2019/1919538

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Copyright © 2019 Tsukasa Kochiyama et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Propofol had no effect on IL-10, TGF-β1, and CD206 gene expression and protein production in M2 macrophages. M0 macrophages were polarized to M2 macrophages in the presence of 0.05% DMSO (solvent control) or propofol (1–5 μM). (a–c) qRT-PCR assays of IL-10, TGF-β1, and CD206 mRNA. Data were normalized relative to β-actin mRNA (internal control) and presented as mean ± SD (n = 3 per group). ELISA measurements of IL-10 (d) and TGF-β1 (e), secreted by M2 macrophages. Data are presented as mean ± SD (n = 3 per group). Statistical comparisons were analyzed using one-way ANOVA with Bonferroni's post hoc test.