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. 2019 Mar 17;2019:1919538. doi: 10.1155/2019/1919538

Figure 6.

Figure 6

siRNA knockdown of Nrf2 significantly reduced the anti-inflammatory effects of propofol in M1 THP-1 cells. (a) Immunoblotting analysis of Nrf2 expression. THP-1 cells were transfected with control nontarget or Nrf2 siRNA and treated with PMA for 72 hr. (b) Densitometric analysis of bands in (a). Data were normalized relative to β-actin (internal control) and presented as mean ± SD of four independent experiments. ∗ P < 0.05 compared with control cells by the Wilcoxon-Mann-Whitney test. (c–e) Effects of Nrf2 siRNA on IL-6, IL-1β, and TNF-α production by M1 THP-1 cells in the absence or presence of propofol. PMA-differentiated siRNA-transfected cells were further polarized into M1 macrophages in the presence of 0.05% DMSO (solvent control) or propofol (50 μM). IL-6, IL-1β, and TNF-α concentrations in supernatants were measured by ELISA. ∗ P < 0.05 compared with cells transfected with nontarget siRNA by one-way ANOVA with Bonferroni's post hoc test. NS = not significant.