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. 2019 Mar 27;12:2235–2245. doi: 10.2147/OTT.S189001

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The effect of TRIB3 on ISK and AN3CA cell growth was evaluated by a CCK-8 assay.

Notes: (A) Western blot analysis showed the ability of TRIB3 overexpression plasmid to upregulate TRIB3 and the ability of TRIB3-shRNA plasmid to downregulate the expression of TRIB3 in ISK and AN3CA cells. ISK and AN3CA cells were transfected with (B and D) TRIB3 overexpression plasmids and (C and E) TRIB3-shRNA plasmids. After 1, 2, and 4 days, the absorbance of cells was measured by CCK-8. NC: TRIB3 overexpression negative control group; T: TRIB3 overexpression group; TI-1: TRIB3-shRNA plasmid 1 group; TI-2: TRIB3-shRNA plasmid 2 group; TINC: TRIB3-shRNA inhibitor negative control group. ^*P<0.05 and ^**P<0.01, compared with the respective control groups by t-test. Data were obtained from three independent experiments.