Figure 2.

Expression of MDM2 and EMT markers in LAC cell lines.

Notes: Human bronchial epithelial-derived BEAS-2B cells were regarded as normal control. The relative mRNA and protein expression levels of E-cadherin, β-catenin, vimentin, N-cadherin, and MDM2 were detected by qRT-PCR (A–E) and Western blot analysis (F and G), respectively. ^*P<0.05, ^**P<0.01, and ^***P<0.001, vs BEAS-2B, respectively. GAPDH was used as reference control.

Abbreviations: EMT, epithelial–mesenchymal transition; LAC, lung adenocarcinoma; MDM2, mouse double minute 2; qRT, quantitative reverse transcription.