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. 2019 Mar 27;12:2247–2258. doi: 10.2147/OTT.S185076

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© 2019 Tang et al. This work is published and licensed by Dove Medical Press Limited

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Effect of MDM2 on EMT progression in LAC cells.

Notes: PC9 and H1975 cells were transfected with MDM2 overexpressing and MDM2-shRNA expressing lentiviruses, respectively. (A–F) The relative expression levels of Snail, Slug, E-cadherin, β-catenin, N-cadherin, and vimentin mRNAs by qRT-PCR analysis. (G) Protein expression of EMT factors in transfected PC9 and H1975 cells by Western blot analysis. GAPDH was used as reference control. (H) Immunofluorescence assay validated levels of E-cadherin and N-cadherin in transfected PC9 and H1975 cells (200× magnification). ^**P<0.01, and ^***P<0.001 in comparison with related control, respectively.

Abbreviations: EMT, epithelial–mesenchymal transition; LAC, lung adenocarcinoma; MDM2, mouse double minute 2; NC, negative control; qRT, quantitative reverse transcription.