Figure 6.

MDM2 promotes tumor growth in in vivo animal tumor model.

Notes: (A–C) The in vivo tumor volume and weight of LAC. Transfected PC9 and H1975 cells were subcutaneously inoculated into male BALB/c nude mice for 42 days. (D–J) The mRNA relative expression levels of MDM2 and EMT-related factors, including Snail, Slug, E-cadherin, β-catenin, N-cadherin, and vimentin. (K, L) The protein expression patterns of MDM2, EMT factors (E-cadherin, β-catenin, N-cadherin, and vimentin), Snail, Slug, and Smad2/3 signaling pathway in tumor tissues. GAPDH was used as control for animal experiments. ^*P<0.05, ^#P<0.05, ^**P<0.01, and ^***P<0.001 in comparison with related control, respectively.

Abbreviations: EMT, epithelial–mesenchymal transition; LAC, lung adenocarcinoma; MDM2, mouse double minute 2; NC, negative control.